Research Results of Shandong Yinfeng Life Science Research Institute on "Stem Cell Promotes New Bone Growth" in Foreign Journals
Large segment bone defect is a common disease in clinic. Stem cells and bone tissue engineering are considered as a promising method for bone regeneration. "Recently, Shandong Yinfeng Research Institute of Life Sciences and Shandong University team cooperated to study a nanocellulosic membrane platform with multi-lineage differentiation of stem cells to construct biologically active three-dimensional bone-like tissue, which can significantly promote the rapid growth of new bone and the rapid formation of capillary and nerve fibers."
Relevant achievements were published in Advanced Healthcare Materials (IF=7.367, JCR Area I) entitled “Nanocellulose-ReinforcedHydroxyapatite Nanobelt Membrane as a Stem Cell Multi-Lineage DifferentiationPlatform for Biomimetic Construction of Bioactive 3D Osteoid Tissue In Vitro”.
Schematic Diagram of Bone Regeneration Multi-differentiation Platform
Massive bone defect is a common clinical disease, especially the treatment of severe bone defect injuries associated with multiple tissue damage (vascular and peripheral nerve injury) and remains a huge challenge, often leading to nonunion and other sequelae and even lifelong disability. Stem cells and bone tissue engineering, as one of the most promising methods of bone regeneration, have attracted widespread attention.
"Most previous studies related to bone tissue engineering scaffolds have focused on osteoblast differentiation and have made great progress," said Wei Benjie, the project's team leader. In fact, bone regeneration involves not only bone formation, but also other parts, such as blood vessels and nerves, to ensure bone function.
In the process of bone repair, if there is no new blood and nerve formation in the new bone, the regeneration of bone defect will fail, resulting in poor prognosis. Therefore, bone tissue engineering for clinical treatment of bone defects should consider the formation of all important parts of bone, in which bone, blood vessels and nerves are particularly important. Because it is difficult to achieve co-culture or multilineage differentiation of different cells in the same scaffold or system, there are few studies on the synergistic regeneration of osteoblasts, vascular cells and neurons. Therefore, developing a multilineage differentiation platform of stem cells to bionically construct three-dimensional bone-like tissue with biological activity remains an urgent problem for bone regeneration.
Reconstruction of Bone-like Tissue in Vitro
It is reported that the hybrid membrane with high mechanical properties and biodegradability was synthesized by assembling ultra-long hydroxyapatite nanobelts in nanocellulosic hydrogels, and the synergistic differentiation and regeneration of stem cells in osteoblasts, nerve cells and vascular endothelial cells were realized. Based on this multi-differentiation characteristic of biomimic hybrid membrane, 3D bone tissue with biological activity was simulated in vitro by laminar superposition, culture and integration.
The 8-week ectopic and in situ osteogenesis experiments showed that the bone-like tissue constructed in vitro significantly promoted the rapid growth of new bone and the rapid formation of capillary and nerve fibers, which proved that it had strong bone regeneration ability. This study shows that hybrid membrane is widely used as a platform for multiline differentiation of stem cells and has wide application prospect in bone tissue engineering.