The research of human cryopreservation is based on the joint research of cryobiomedicine, cell biology and clinical medicine, which can slow down or even suspend the metabolic rate of human cells, tissues and organs, block the process of function failure and "biological death", protect the integrity of cell structure and the function of biochemical pathway, realize the suspension of physiological and biochemical functions of human tissues and organs, and make life last. In 2017, the operation of the first human cryopreservation volunteer in Asia was completed.
Original minimally invasive surgical protocol: Minimally invasive dual access extracorporeal circulation perfusion and femoral arteriovenous ECMO percutaneous perfusion technique. This technique avoids the open mechanical injury to the body caused by thoracotomy. It is punctured and drained through femoral artery, vein and bilateral external jugular vein of neck, and connected with cardiopulmonary bypass equipment, which not only ensures good circulatory perfusion of the whole body but also focuses on the brain, thus improving the perfusion efficiency. Under standard conditions, cardiopulmonary bypass is established within 30 minutes, and the in vivo core temperature can be reduced to not more than 18°C at the end of the cycle by combined in vitro and in vivo cooling techniques.
Independent research and development of cryoprotectant: YF-CS-01 cryoprotectant for human cryopreservation independently developed by our hospital, and 6 concentrations of cryoprotectant are used for gradient systemic perfusion to ensure the gradual penetration and adaptation of body tissue cells to cryoprotectant. At the end of perfusion, the core temperature of the body can be maintained at 4-10°C, and the concentration of cryoprotectant in the circulatory system can reach 65-70%.
High-precision and long-span program cooling instrument: Yinfeng Research Institute has developed the world's first high-precision and large-span human body low-temperature preservation program cooling equipment. The equipment is equipped with more than ten electromagnetic valves and temperature sensors. Each solenoid valve corresponds to a U-shaped liquid nitrogen tube, and each liquid nitrogen tube is designed with many small holes of different angles, which can make the liquid nitrogen become more uniform and rapid. Then, the liquid nitrogen vapor is controlled by precise linkage control of computer program, and the liquid nitrogen vapor jet is ingeniously used to rapidly form liquid nitrogen vapor vortex in the cooling chamber. Ensure a rapid and balanced cooling of the sample. After 50 - 60 hours of cooling, the body can cool down to -190°C evenly and continuously to ensure that the body is protected from injury during the cooling process.
Independently developed cryosurgical perfusion monitoring system: In order to more accurately, real-timely and intuitively monitor the key technical indicators such as temperature, pressure, flow rate and degree of protection agent replacement in the life continuation research program, our hospital has invested a lot of human and financial resources, and spent 10 months in the association with the scientific research organization to complete the development and system optimization of the cryosurgical perfusion monitoring system. The comprehensive performance of the system has been verified for many times through experiments, and has now been applied to the implementation of the life continuity plan.
Customized cryopreservation equipment: Our hospital adopts customizable liquid nitrogen storage tanks for two and four persons to meet the long-term storage and use needs of different volunteers. Liquid nitrogen tank adopts liquid level display + multi-point temperature dynamic detection, equipped with independent liquid level monitoring instrument and network liquid level monitoring, which can realize automatic replenishment of liquid nitrogen, automatic alarm for abnormal conditions and high degree of safety.
Humanized value-added service: Our hospital provides 1000mL peripheral blood gradient density centrifugation for each volunteer participating in the life-continuation program to obtain mononuclear cells (MNC) for simultaneous programmed cooling and cryopreservation, providing necessary seed cells for volunteer resuscitation research.