Compared to the commonly used tranditional thoracotomy, the minimally invasive dual-access operation protocol combined with the extracorporeal circulation is more minimally invasive, precise, and highly efficient. By establishing two circulatory pathways, through the jugular and femoral arteries/veins, this protocol enables simultaneous cryoprotectant perfusion to both the brain and the entire body, which significantly reduces surgical trauma while improving perfusion efficiency and quality, and at the same time, optimizes the effect of the protection of the brain.The unique design ensures more uniform perfusion and effective protection for the brain and systemic organs during the operation, effectively preventing ischemic brain injuries caused by the traditional approach. This breakthrough substantially enhances protection efficacy during cryopreservation. Additionally, the surgical incision size is reduced from 30 cm to 3-5 cm, markedly minimizing trauma for participants to lay a solid technical foundation for subsequent rewarming researches.

Our research and development team at Yinfeng Life Science Research Institute is dedicated to developing cryoprotectants that are highly efficient, green and easy to apply, and continues to optimize the formulation of the protectants and push forward the technological iteration. At present, our team has independently developed over 30 types of YF-series cryoprotectants covering cells, tissues, organs, and other fields. Notably, the cryoprotectant for large-scale intact organisms has been upgraded to version 3.0.In terms of customized development, experimental validation, patented technology and application effect, YF series cryoprotectants have demonstrated significant advantages in the cryopreservation of many different types of biological samples, especially in the field of large-size intact organism cryopreservation, which has an important technological status and development potential.

In 2016, the Yinfeng Life Science Research Institute successfully developed the world’s first high-precision temperature-controlled cooling device based on air vortex principles, specifically designed for the cooling of severed limbs and large-scale intact organisms. This breakthrough filled a global technological gap in the field. The device integrates an advanced big data model that automatically generates cooling protocols based on critical parameters such as perfusion status, volume, and weight of biological samples. In addition, innovatively, the device achieves active control of the ice crystal formation and growth process by setting specific physical field conditions, which effectively reduces the damage of ice crystals to biological samples. Equipped with 11 high-precision temperature sensors inside the device, the device can monitor and provide real-time feedback on temperature fluctuations, ensuring precise control of the cooling module and achieving stable and continuous cooling from room temperature to -190°C. With its excellent performance and innovative technology, the device was successfully exported to research institutes in Australia in 2021, gaining international recognition.

Building on Extracorporeal Membrane Oxygenation (ECMO) technology, Yinfeng Life Science Research Institute has innovatively developed an advanced perfusion and monitoring system with independent intellectual property rights for the key technical indexes such as temperature, pressure, flow rate, and cryoprotectant exchange. This system not only significantly enhances the perfusion efficiency but also realizes real-time visualization of parameters. Integrated with the feedback control algorithms, it substantially improves the precision and reliability of operation. Furthermore, the institute has optimized the system’s piping materials for low-temperature resistance, greatly enhancing the stability of consumables under cryogenic conditions. These innovations effectively shorten the time required for cryoprotectant loading and removal, significantly reduce the toxicity damage to biological samples, and further enhance the overall efficacy and safety of cryopreservation.

The ecosystem independently developed by Shandong Yinfeng Life Science Research Institute for long-term cryopreservation of large-sized intact organisms has demonstrated significant leading advantages by virtue of its excellent integrated design and innovative technology, which guarantees the quality of cryopreservation of samples by ultra-low temperature in an all-round way.This system not only incorporates foundational functions such as automatic liquid nitrogen replenishment, vacuum adiabatic function for equipment, and dynamic monitoring of critical parameters (temperature, liquid level, pressure, and oxygen concentration), but also innovatively introduces intelligent and safety-enhancing modules, including infrared and remote security alarms, automatic power supply switching, quality control calibration and inspection, and database management.By integrating these features, the system guarantees the stability of specimens in ultra-low-temperature environments while substantially enhances operational reliability and user-friendliness, realizing the fine control of the whole process from sample preservation to data management.

With our excellent resources in the fields of cells, genes and health management, we have crafted diversified value-added services for our members and participants in order to help the Yinfeng Life Extension Program. For participants, we could provide peripheral blood mononuclear cell (PBMC) preservation and stem cell colonization technologies to reserve critical seed cells for future rewarming research. Additionally, we would offer whole-genome sequencing and high-resolution HLA typing services to precisely preserve their biological data.In addition, we also provide members, participants and their families with the life-cycle health management services. This is not only the respect and return to the selfless contribution of members and participants we can provide, but also provides strong support for the scientific research and clinical transformation of cryopreservation technology, which is ofscientific value, humanistic care and social significance.