-01 Application of Minimally Invasive Surgical Approach

Our minimally invasive surgical approach leverages a dual-channel extracorporeal circulation infusion, enabling the perfusion of cryoprotectants via two circulatory routes-the cervical artery and vein, and the femoral artery and vein, thereby concurrently safeguarding the human brain and various body organs and enhancing perfusion quality. Unlike foreign craniotomy and thoracotomy surgical schemes, our minimally invasive surgical approach minimizes surgical trauma to the volunteers. Compared with the traditional perfusion method that requires thoracotomy to perform cryoprotectants on the human body, it not only reduces wound formation but also ensures that cryoprotectants can quickly and fully reach all tissues of the body, which lays a solid technical foundation for future human tissue and organ resuscitation tests.

-02 Self-developed Cryoprotectant

Cryoprotectants are indispensable components in the low temperature preservation/deep cryopreservation of cells, tissues, and organs to reduce freezing damage during perfusion. As early as 2016, our institute began exploring deep cryopreservation with the Hand and Foot Surgery Department of Qilu Hospital of Shandong University. As of the end of December 2023, we have successfully performed deep cryopreservation of detached skin from 85 patients, with successful replantation in 25 cases, achieving good transplant results and gradually establishing a standard process for clinical deep cryopreservation of detached skin. At present, our institute have developed more than 30 kinds of cryoprotectants for different types of cells, tissues, and organs, establishing the YF series of low temperature preservation/deep cryopreservation systems. This provides cryoprotectant screening, cooling/rewarming technical guidance for different types of biological samples, offering high-quality services and support for the deep cryopreservation of cells, tissues, organs, and human health.

-03 High-precision Programmed Cooling Equipment

In 2016, our institute developed the world’s first high-precision low-temperature programmed cooling equipment based on the principle of air vortex. Currently, this programmed cooling equipment is the largest in the world, with the widest temperature control range and highest precision. It fills the global technical gap of programmed cooling instruments from room temperature to -196℃ in a continuous curve and solves the demand for large-size cooling instruments for various types of large-volume tissues, organs and human body preservation. It is exported and circulated worldwide, and was exported to Australia in 2021, demonstrating China’s scientific and technological strength in the cryobiological domain.

-04 Self-developed Cryosurgery Perfusion Monitoring System

To more accurately, real-time, and intuitively monitor key technical indicators such as temperature, pressure, flow, and cryoprotectant replacement degree during hypothermic perfusion and surgery, our institute spent 10 months to complete the research and development and system optimization of the cryosurgery perfusion monitoring system, and verified the comprehensive performance of the system many times through experiments. It has now been maturely applied to the clinical rewarming of various tissues and organs and human body cryopreservation and perfusion surgery.

-05 Customized Cryopreservation Equipment

Our deep cryopreservation equipment adopts liquid level display + multi-point temperature dynamic monitoring, equipped with independent liquid level monitoring instrument and network liquid level monitoring. It can realize intelligent liquid nitrogen replenishment, automatic response to abnormal conditions, and other functions, comprehensively ensuring the quality of volunteer’s deep cryopreservation.

-06 Humanized Derivative Services

To provide more support and possibilities for future human resuscitation experiments, we offer volunteers a number of derivative services:
1. These include providing volunteers with 1000 mL peripheral blood density gradient centrifugation technology service to obtain mononuclear cells (MNC), which provide necessary seed cells for their resuscitation research.
2. During the perfusion surgery, we can provide volunteers with stem cell colonization, which permeates into the volunteers’ body with the infusion of the cryoprotectant, thereby better protecting the tissues and organs of the volunteer’s body. 
3. We also offer whole genome sequencing, high-resolution HLA matching detection, applying the world’s most advanced gene technology to the human body cryopreservation project.