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A Standard Protocol for Cryopreservation of Intact Organisms

Initial consultation

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Consult with staff on how to become a participant of our program and other related issues via official phone number and customer service on WeChat.

Site visits and tours

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Make an appointment with the staff in advance for a site visit and a tour to the base of the program and a detailed interview with the staff.

Application submission

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Prospective participants or their authorized representatives complete the following documents:
Shandong Provincial Body (Cornea) Donation Registration Application Form,Yinfeng Life Extension Program Participant Application Form.

Evaluation and review

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Shandong Yinfeng Life Science Research Institute evaluates whether prospective participants meet the conditions for program recruitment from the dimensions of legal ethics, medical conditions, and implementation plan.

Approval

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Participant applications are formally approved by the institute.

Transportation (for non-Jinan residents)

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In order to improve the communication efficiency and clinical responsiveness of individuals participating in the Program and to maximize the effectiveness of the implementation, participants are encouraged to transport to hospitals/hospice care facilities in Jinan, Shandong, during critical illness or prior to end stages.

Clinical Response

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Medication support and cardiopulmonary support to ensure blood and oxygen supply to the participant's body after clinical death.

Cryoprotectant agents perfusion

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The replacement of cryoprotectant agents and blood using a minimally invasive dual-access extracorporeal circulation surgical protocol.

Programmed cooling

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Use the high-precision temperature-controlled cooling device to cool down the participant’s core temperature to -190°C.

Cryopreservation

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Transport the participant to a cryopreservation system of -196°C for long-term storage.