Focus on Frigid Zone Medicine: Yinfeng Low - Temperature Medical Team Publishes an Important Review on Cryopreservation
Release time:
2025-08-29
Recently, Frigid Zone Medicine, an authoritative international journal in the field of cryomedicine, published an important review titled "Advances in the Detection Methods for Assessing the Viability of Cryopreserved Samples". Written by the team of Yinfeng Cryomedical Research Center, the article systematically reviews and analyzes various detection techniques currently used to evaluate the viability of cryopreserved cells, tissues, and organs. It also proposes key directions from the perspectives of methodological integration and future instrument development, offering crucial theoretical support and practical guidance for the long - term cryopreservation of complex tissues and organs.
Cryopreservation: The "Time - Pausing" Technology in Medicine
As a branch of biology that studies the effects of low temperatures on organisms and their applications, cryobiology has been increasingly applied in medicine in recent years. In particular, fields such as organ transplantation, reproductive medicine, and tissue engineering have a growing demand for long - term and efficient preservation of biological samples. Although significant progress has been made in the cryopreservation of cells and simple tissues, the cryopreservation of large organs like the heart, liver, and kidneys still faces huge challenges. Problems such as ice crystal damage, cryoprotectant toxicity, oxidative stress, and rewarming damage remain the main obstacles to long - term organ preservation.
Cryogenic Research Urgently Needs a Systematic Evaluation of the Viability of Frozen Samples
In this context, a series of evaluation methods must be used to detect damage at each stage to comprehensively understand the effects of freezing and rewarming on cells, tissues, and organs. Although current technologies can detect damage to some extent, they are often relatively simple, time - consuming, and have limited ability to provide timely and comprehensive feasibility assessments. Therefore, optimizing existing processes and improving damage detection strategies are key steps in advancing cryopreservation technology. Investigating the degree of damage caused by different factors at the cellular, tissue, and organ levels is very important for improving preservation protocols and overall results.
From Cells to Organs: A Panoramic Analysis of Multi - Dimensional Viability Detection Technologies
The article details the common viability assessment methods used in current research on damage detection and damage mechanisms, including:
Macroscopic Appearance and Shape Evaluation: For example, surgeons intuitively judge liver viability by color, texture, etc. Although it is simple, it is highly subjective, and additional indicators and more refined methods are needed to accurately evaluate organ viability.
Cell Morphology Detection: Such as hematoxylin and eosin (H & E) staining, trypan blue exclusion test, Nissl staining, etc., which can reflect cell morphology and the degree of loss to some extent.
Cell Apoptosis Detection Tests: Such as the annexin V/propidium iodide method, terminal deoxynucleotidyl transferase dUTP nick - end labeling (TUNEL) assay, caspase - 3 activity assay, lactate dehydrogenase (LDH) release assay, etc., which are used to evaluate the mechanism and degree of cell death during the cryopreservation and rewarming of tissues and organs.
Tissue Biomarker Detection: Using various enzymes essential for the metabolic process as indicators to evaluate viability.
Cell Viability Assays: Such as 3 - (4,5 - dimethylthiazol - 2 - yl) - 2,5 - diphenyltetrazolium bromide (MTT) assay, neutral red uptake assay, Western blot analysis of β - actin, calcein - AM cell viability assay, etc., which are widely used in various cell viability determinations.
Tissue Viability Assays: Determination of ATP content, glucose uptake assay, etc., which provide indicators of the metabolic activity and overall viability of various cell types.
These methods provide multi - dimensional insights into the degree of cryogenic damage at the cellular and tissue levels. Each has its advantages but also limitations. There is an urgent need to develop more quantitative, scalable, and integrated tools for viability assessment in cryopreservation research.
Research Significance: Laying the Foundation for the Next - Generation Cryopreservation Technology
This review not only summarizes existing technologies but also provides clear guidance and forward - looking perspectives:
Promote the Development of Detection Methods from "Single - Indicator" to "Multi - Dimensional Integration": The article emphasizes that in the future, integrated platforms capable of simultaneously evaluating morphology, metabolism, function, and molecular levels should be developed to achieve a systematic assessment of sample viability.
Facilitate the Application of Real - Time and Non - Destructive Detection Technologies: For example, embedding biosensors in perfusion systems to enable real - time monitoring of viability indicators during organ preservation, thus avoiding the common problem of sample destruction in traditional methods.
Support Clinical Translation and Application Expansion: A reliable viability assessment system is the core prerequisite for the clinical application of organ cryopreservation. This review provides a methodological basis for subsequent research on the low - temperature resuscitation of large organs and is expected to greatly promote the development of transplantation medicine, regenerative medicine, and space biomedicine.
The systematic publication of this review not only enhances the scientificity and comprehensiveness of the viability assessment of cryopreserved samples but also points the way for subsequent technological research and development and clinical applications. It is expected to accelerate the process of translating cryomedicine from the laboratory to clinical practice.
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Recently, Frigid Zone Medicine, an authoritative international journal in the field of cryomedicine, published an important review titled "Advances in the Detection Methods for Assessing the Viability of Cryopreserved Samples". Written by the team of Yinfeng Cryomedical Research Center, the article systematically reviews and analyzes various detection techniques currently used to evaluate the viability of cryopreserved cells, tissues, and organs. It also proposes key directions from the perspectives of methodological integration and future instrument development, offering crucial theoretical support and practical guidance for the long - term cryopreservation of complex tissues and organs.
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