Pay tribute to the heroes behind the scenes - using experimental data to support the exploration of life sciences

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Research in basic science is supported by experimental data one by one, and the same is true for exploration in life sciences. The generation of every experimental data is inseparable from the contribution and dedication of experimental animals, and they are all precious.

On the morning of August 28, 2021, the Cryomedicine Research Center of Shandong Yinfeng Life Sciences Research Institute, the research unit of our foundation, welcomed two special "volunteers": female beagles named Cryobig8 and Cryobig9 respectively. The eighth and ninth animal "volunteers" to participate in the experiment since the launch of the Yinfeng Life Extension Project. In order to more accurately and intuitively monitor key technical indicators such as temperature, pressure, flow rate and protective agent replacement degree during cryopreservation surgery and perfusion, as well as the operation of the cooling program, Cryobig8 and Cryobig9 will be verified through different cryopreservation experimental surgeries.


Use experiments to verify the scientific nature of surgery



At 10:00 a.m., cryogenic surgery officially began. After Cryobig8 was anesthetized, surgical experts established an extracorporeal circulation-connected cryogenic surgical perfusion detection system for him, turned on the extracorporeal circulation machine and membrane lung, and quickly lowered his body temperature to below 18°C through a low-temperature circulating water bath, and quickly performed basic perfusion fluid and Cyclic perfusion of cryoprotectants with different concentrations. As a control experiment, Cryobig9's perfusion method was different, using only its own blood for low-temperature circulation cooling.

After the perfusion was completed, the experimental dogs were transferred to a programmed cooling device that was pre-cooled to 4°C. The programmed cooling was started. After more than 20 hours, the temperature was reduced to -190°C, and then transferred to an independent stainless steel liquid nitrogen storage tank for liquid phase storage.


perfusion surgery

The surgery and perfusion lasted from 10 am to 17 pm on the 4th, which took 7 hours. A team of clinical experts including cardiac surgery experts Sun Wenyu and Zhao Xin, anesthesia experts Hou Yuedong and Li Huihui, and extracorporeal circulation specialist Zhao Fen participated in the demonstration and implementation of the experimental plan.


In order to verify the protective effect of the cryoprotective solution on the body and tissues and organs, after the perfusion was completed, some kidneys, livers and muscle tissues from different parts of the experimental period were taken as samples, and the frozen samples were used to analyze tissue cell morphology, activity, etc. detection.


According to the predetermined experimental plan, the two experimental dogs will also undergo brain CT scans in the future to compare and analyze the perfusion effect of cryoprotectant and the structural changes of tissues and organs after preservation.

Tribute to the heroes behind the scenes

Beagles are recognized by the international medical and biological communities as the most ideal experimental dog breed. Because their internal organs are very similar to humans, and they have good physical fitness and a strong sense of obedience, they have become the best choice for in vivo experiments among many animals. They are warriors in peaceful times who have made great sacrifices for our human health and the development of life sciences.


Since the launch of the Yinfeng Life Extension Research Plan in 2015, the institute has conducted multiple animal experiments to simulate surgical procedures, verify perfusion effects, improve surgical plans, etc. The Yinfeng Life Extension Plan is based on the continuous exploration of researchers. , with the dedication of these "behind-the-scenes heroes", they have pioneered research and achieved historic breakthroughs one after another, which has enabled my country's cryopreservation technology to continuously improve and rival the world.

Finally, let us pay tribute to the beagle! Pay tribute to these behind-the-scenes heroes who have made great contributions to the front line of scientific research! Pay tribute to all the unknown soldiers who have sacrificed their precious lives for the development of human health and medicine!

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